Facilities

The Phosphorimager Core Facility, located in room A-022, consists of a Typhoon Triad. This instrument is capable of scanning storage phosphor screens that have been exposed to radioactive samples as well as chemiluminescence and direct red, blue or green fluorescence. In addition to storage screens, blots and gels can be scanned directly on the Typhoon which can also be used to scan a wider variety of objects, including microtiter plates, tissue sections and microarrays.

Applications include acquiring images of 1D or 2D gels, blots, tissue sections, etc. using either storage phosphor screens, chemiluminescence or specific fluorescent stains excited by red (633 nm), green (532 nm) or blue (488 or 457 nm) light. Protein or nucleic acid determination can also be done in microtiter plates when stained with relevant fluorescent dyes.

Data from any of the formats and either machine can be quantitated using available ImageQuant software.